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. 2021 Jul 15;13(7):7556–7573.

Figure 3.

Figure 3

CircRPPH1 directly interacts with miR-296-5p. A: qRT-PCR for miR-296-5p level in 70 paired BC and surrounding normal tissues; B: miR-296-5p expression in multiple BC cells was assessed by qRT-PCR; C: qRT-PCR for assessing expression of circRPPH1 in the nuclear and cytoplasmic fractions of BC cells; D: MiR-296-5p was predicted as a target of circRPPH1 by Circatlas, CircBank, and StarBase databases; E: The putative binding sites between circRPPH1 and miR-296-5p are shown; F: The direct binding between circRPPH1 and miR-296-5p was confirmed by dual luciferase assay; G: RNA pull-down assay; H: RIP assay; I: qRT-PCR for miR-296-5p expression in BC cells after silencing of circRPPH1. Data are shown as mean ± standard deviation, ***P<0.001.