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. 2021 Jul 15;13(7):7556–7573.

Figure 5.

Figure 5

miR-296-5p directly targets FOXP4 in BC cells. A: ZCCHC3, FOXP4, and CCAR2 were predicted as target genes of miR-296-5p using RNA22, Targetscan, and Pictar databases; B: qRT-PCR for the mRNA expression of ZCCHC3, FOXP4, and CCAR2 in miR-296-5p-overexpressed BC cells; C: The mRNA expression of FOXP4 in 70 paired BC and surrounding normal tissues was determined by qRT-PCR; D: FOXP4 mRNA expression in various BC cells was detected by qRT-PCR; E: The putative binding sites between FOXP4 and miR-296-5p are shown; F: Dual luciferase assay for evaluating the interaction between FOXP4 and miR-296-5p; G: Western blotting for detecting FOXP4 protein abundance in miR-296-5p-overexpressed BC cells; H, I: FOXP4 expression in BC cells from different groups was assessed by qRT-PCR and Western blotting, respectively. Data are shown as mean ± standard deviation, **P<0.01, ***P<0.001.