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. Author manuscript; available in PMC: 2021 Aug 5.
Published in final edited form as: Biochemistry. 2021 Jan 7;60(2):118–124. doi: 10.1021/acs.biochem.0c00917

Table 1.

Steady-State Kinetic Constants for Native AP, [2H]AP, and [2H,13C,15N]AP for p-Nitrophenyl Phosphate (pNPP) and 4-Methylumbelliferyl Phosphate (4-MUP) Substrates

native EcAP [2H]EcAP [2H,13C,15N]EcAP
pNPP
 kcat (s−1) 7.6 ± 0.2 8.0 ± 0.2 7.6 ± 0.2
 Km (μM) 7.4 ± 0.7 9.9 ± 0.9 10.3 ± 0.8
 kcat/Km (M−1 s−1) (1.0 ± 0.1) × 106 (0.80 ± 0.2) × 106 (0.7 ± 0.1) × 106
4-MUP
 kcat (s−1) 13.1 ± 0.5 13.2 ± 0.6 12.9 ± 0.4
 Km (μM) 11.4 ± 1.0 13.9 ± 1.8 11.4 ± 0.9
 kcat/Km (M−1 s−1) (1.1 ± 0.2) × 106 (0.9 ± 0.3) × 106 (1.1 ± 0.2) × 106
 kobs (s−1) 524 ± 3 515 ± 2 501 ± 2