Fig. 2.

Tumor-infiltrating leukocyte populations differ significantly in the different versions of the MMTV-PyMT breast tumor model. Tumors from the autochthonous MMTV-PyMT model were harvested and single-cell suspensions were generated. Cells (1E6, 1E5, or 1E4) were injected into the mammary fat pad of FVB/NJ wild-type mice. When the tumors reached 100 mm³, tumors were obtained and processed into a single-cell suspension for immunophenotyping by flow cytometry. a Analysis comparing the absolute number of lymphocytes (CD3⁺) and lymphocyte subsets (CD8⁺ and CD4⁺) per gram of tissue. b Analysis comparing the frequency of lymphocytes (CD3⁺) and lymphocyte subsets (CD4⁺ and CD8⁺) out of total CD45⁺ cells. c Analysis comparing the absolute number of myeloid cells (CD11b⁺) and myeloid cell subsets (F4/80⁺ and Gr1⁺CD11b⁺) per gram of tissue. d Analysis of myeloid cell subsets as a percent of total CD45⁺ cells. Graphs show mean ±SEM from at least two independent experiments with at least three mice per group. Each data point represents an individual mouse. Unpaired two-tailed t test. *P<0.05, **P<0.01, *** P<0.001, and **** P<0.001