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(a) Repeated detection of 5 pM miR-34a with the same microfluidic device while the SERS substrates were changed upon every detection time. (b) Representative Raman spectra of the SERS substrate during repeated detection of 5 pM miR-34a after washing the microfluidic device and reunion with the MB-modified SERS substrate. Analysis of the SERS substrate was performed using a 785 nm laser at 50 mW for 10 s.
