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Experimental setup for the inflation test of the human LC and PPS of the same eye showing the a) ONH cut flush to the sclera; b) ring-mounted posterior scleral cup; c) custom inflation holder; d) Zeiss LSM 710; e) the maximum intensity projection of the SHG image volume and f) of the TPF image volume for specimen Specimen 2; and g) the imaging setup with components labeled.
