Fig. 8.

Role of circ-SCMH1 in DDP resistance of OSCC cells in vivo. Xenograft tumor models were established using SCC-15/DDP cells stably transfected with shRNA against circ-SCMH1 (sh-circ-SCMH1) or the negative control or (sh-NC), and then treated with DDP (3 mg/kg) or equal volume of phosphate-buffered saline (PBS). A, B Tumor volume and weight were measured using caliper and electronic balance. C RT-qPCR measured circ-SCMH1 and miR-338-3p levels in tumor tissues of xenograft mice with normalization to GAPDH and U6, respectively. D Western blotting measured LIN28B and GAPDH protein level in tumor tissues of xenograft mice. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 from three separate assays