Fig. 2.

Map-based cloning of BnDWARF2. a The BnDWARF2 locus was mapped primarily on C04 chromosome between the SNP markers M33367 and M35244. b The BnDWARF2 locus was fine-mapped in the 34.62 kb region between SSR markers S3 and S4. The numerals indicate the number of recombinants. c The genes in the mapping interval. d The gene structure and the mutation sites in BnaC04.BIL1. e The protein structure and the mutation sites of the BnaC04.BIL1 protein, and the STKc_GSK3 superfamily domain was predicted. Solid lines show the position of the amino acid transition. f The chromatogram of BnaC04.BIL1 at 1565–1585 bp in ZS11 and Bndwarf2 mutant, respectively. g The chromatogram of BnaC04.BIL1 at 2900–2920 bp in ZS11 and Bndwarf2 mutant, respectively. The black arrows denote the A1575T and G2910T substitutions, respectively