FIGURE 5.

ACAP2 increased hypoxia-induced apoptosis of AC16 cells through miR-532. The effects of transfections on the apoptosis of AC16 cells induced by hypoxic treatment (1% O₂, 5% CO₂, and 94% N₂) for further 48 hours were analyzed by cell apoptosis assay, which was performed by annexin V-FITC and PI staining and flow cytometry. Each cell apoptosis experiment was performed in 3 biological replicates, and values presented here were mean ± SD values. The arbitrary unit was used for all values. *P < 0.05.