Fig. 3.

Human brain biopsy (cortex). A 243 μm × 243 μm × 104 μm volume is shown embedded in a 1 cm bulk sample. (a) Enlarged selected region from (b) a representative en face slice at 48 μm depth. Scale bar is 50 μm. (b) From left to right, arrows indicate axonal cross section, extracellular vesicles, and neuronal cell nucleus, respectively. Scale bar is 20 μm. (c) Side central cross section at the yellow dashed line in (b), at the same scale as (b). (d) Enlarged selected region from (c). From left to right, the images represent the same region from a conventional OBM reconstruction, 2D qOBM, and 3D qOBM reconstructions, respectively. Arrows indicate a pooled red blood cell (top) and an axially traversing axon cross section. Scale bar is 25 μm. (e) Shannon entropy of image as function of depth.