Fig. 4. The structures of representative ABPP probes applied in state-of-the-art chemoproteomic strategies. (A) Iodoacetamide-alkyne is a widely applied cysteine-reactive ABPP probes.54 (B) NHS-ester-alkyne is a versatile covalent ABPP probe for protein nucleophiles (cysteines, lysines, tyrosines, serines, threonines, and arginines).65 (C) STP-alkyne is an amine-reactive covalent ABPP probe.67 (D) Latent electrophiles as exemplified by SuFEx-based arylfluorosulfates for lysine/tyrosine targeting.72 (E) HHS-465 and HHS-475 are SuTEx-chemistry based ABPP probes for tyrosine profiling.74 (F) Carbon nucleophiles help to explore ligandability of sulfenylated (oxidized) cysteines.75.
