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. Author manuscript; available in PMC: 2021 Aug 5.
Published in final edited form as: Mol Pharm. 2015 Jun 9;12(7):2544–2550. doi: 10.1021/acs.molpharmaceut.5b00257

Scheme 1. Production of a cpAb or cp-bsAb by Aldolase Ab Programming (AACP) Strategy Using a Fully Functionalized Antibody-Programming Agent (Ab-PA) or Precursorsa.

Scheme 1.

aAb PA or a functionalized linker (* stands for two different linker-functional groups) that can react covalently through a β-lactam (L) function with reactive lysine residues in the aldolase Ab binding sites yielding cpAb, cp-bsAb, and cpAb intermediates. CpAb intermediates further react with compounds (comp1/2) affording a cpAb or cp-bsAb. Linkers and Ab PAs can also react with Ab through the β-diketone (DK) or vinylketone (VK) function. (Ab, antibody. PA, programming agent: a compound fully functionalized with lactam, vinyl ketone, or diketone linker. LNK, linker. FG, functional group.).