|
Exonuclease activity |
| Phosphodiesterase I from snake venom phosphodiesterase (SVPDE) |
5′ exonuclease targeting ss or ds DNA or RNA. 3′ → 5′ activity |
| Calf spleen phosphodiesterase (CSPDE) |
3′ exonuclease targeting 5′-OH ss or ds DNA or RNA. 3′ → 5′ activity |
| Phosphodiesterase II from bovine spleen (spleen phosphodiesterase) |
3′ exonuclease targeting 5′-OH ss or ds DNA or RNA. 5′ → 3′ activity |
| Exonuclease III |
5′ exonuclease targeting ds DNA/DNA or DNA/RNA. 3′ → 5′ activity |
| Calf intestinal alkaline phosphatase (CIAP) |
Catalyses non-specific dephosphorylation at the 3′ and 5′ ends of a DNA/RNA strand |
| T4 polymerase digestion |
5′ exonuclease targeting ds DNA. 3′ → 5′ activity |
|
Endonuclease activity |
| DNase I |
Hydrolyses ss or ds DNA producing 3′-OH and 5′-P (preferably takes place at a position adjacent to a pyrimidine) |
| Nuclease P1 |
3′ → 5′ activity targeting ss DNA or RNA |
| Nuclease S1 |
Hydrolyses ss or ds DNA or RNA |
| RNase-H |
Hydrolyses the RNA strand of a hybrid DNA/RNA duplex |
| RNase-A |
Hydrolyses ss RNA |
|
Digestion activity |
| EcoRi 1 |
Specifically recognizes the palindromic G/AATTC sequence of a DNA duplex |
| Endonuclease Nsi1 |
Specifically recognizes the palindromic ATGCA/T sequence of a DNA duplex |
