. 2020 Dec 8;2(1):94–150. doi: 10.1039/d0cb00136h

Thermal denaturation studies (T_m values) of the chimeric R_p and S_p-AEP/PO-ODN used with complementary single-stranded DNA or RNA and their half-life evaluations against nuclease S1¹⁹¹.

ODN (5′ → 3′)^a	T _m with DNA (°C)	T _m with RNA (°C)	t _1/2	RNase-H activation
ODN (5′ → 3′)^a	T _m with DNA (°C)	T _m with RNA (°C)	Nuclease S1	RNase-H activation
d(T₁₃)	34	30	4 min	✓
[d(T_AEPTT_AEPTT_AEP-TT_AEPTT_AEPTT_AEPTT)]-all-R_p	51	35	>24 h	✗
[d(T_AEPTT_AEPTT_AEPTT_AEPTT_AEPTT_AEPTT)]-all-S_p	12	—	>24 h	✗

AEP refers to a (2-aminomethyl)phosphonate internucleoside linkage.

Thermal denaturation studies (Tm values) of the chimeric Rp and Sp-AEP/PO-ODN used with complementary single-stranded DNA or RNA and their half-life evaluations against nuclease S1191.