Half-life evaluations of AcPO- and AcPS-ODN against SVPDE209 and initial rates for RNase-H210.
| ODN (5′ → 3′)a | t 1/2 b | Initial rate (min−1) |
|---|---|---|
| d(CTCAAGTGGGCTGGTGAC)-all-PO | <15 min | 0.577 |
| d(CTCAAGTGGGCTGGTGAC)-all-PS | >18 h | 0.244 |
| d(CTCAAGTGGGCTGGTGAC)-all-AcPO | >18 h | 0.000522 |
| d(CTCAAGTGGGCTGGTGAC)-all-AcPS | >18 h | 0.0134 |
| d(CAcPSTAcPOCAcPOAAcPOAGTGG | — | 1.421 |
| GCTGGAcPOTAcPOGAcPOAAcPOC) | ||
| d(CAcPSTAcPSCAcPSAAcPSAGTGG | — | 1.266 |
| GCTGGAcPSTAcPSGAcPSAAcPSC) |
a
PO, PS, AcPO and AcPS refer to the phosphodiester, phosphorothioate, phosphonoacetate and thiophosphonoacetate internucleoside linkages respectively.
b
ODN not tested.