. 2020 Dec 8;2(1):94–150. doi: 10.1039/d0cb00136h

Thermal denaturation studies (T_m values) of PS-ODN with complementary RNA and their half-life evaluations against SVPDE, Pol I and fetal calf serum (FCS)⁷⁹.

ODN (5′ → 3′)^a	T _m with RNA (°C)	t _1/2 ^b			RNase-H activation
ODN (5′ → 3′)^a	T _m with RNA (°C)	SVPDE (s)	Pol I (min)	FCS (h)	RNase-H activation
[d(CTCTCGCACCCATCTCTCTCCTTCT)]-all-PO	73.1	88	30	—	✓
Hairpin-all-PO^c	72	>1000	>120	—	✓
[d(CTCTCGCACCCATCTCTCTCCTTCT)]-all-PS	65	—	120	≫16	✓
Hairpin-all-PS^c	63	—	>240	∼4	✓

PS and PO refer to the phosphorothioate and phosphodiester internucleoside linkages respectively.

ODN not tested.

Hairpin loop structure of the ODN studied.

Thermal denaturation studies (Tm values) of PS-ODN with complementary RNA and their half-life evaluations against SVPDE, Pol I and fetal calf serum (FCS)79.