Half-life evaluations of different Me-SATE-ODN against PLE, SVPDE, CSPDE, TCE, human serum and human gastric juice235.
| ODN (5′ → 3′)a | t 1/2 | |||||
|---|---|---|---|---|---|---|
| SVPDEb (h) | CSPDEb (h) | PLEc | TCEb,c,d (h) | Human serumc (h) | Human gastric juicee (d) | |
| d(T)10-all-Me-SATE-PO-ODN | >19 | >28 | 4.6 h | 0.35 (22) | 3 | >7 |
| d(T)10-all-Me-SATE-PS-ODN | >19 | >28 | >2 d | 9.7 (20) | >24 | >7 |
a
Me-SATE-PO-ODN and Me-SATE-PS-ODN refer to Me-SATE phosphate and thionophosphate internucleoside linkages respectively.
b
Nuclease activity.
c
Carboxyesterase activity.
d
Values in brackets correspond to the incubation times necessary for the formation of 50% of fully deprotected ODN (carboxyesterase activity).
e
Acidic medium stability.