Thermal denaturation studies (Tm values) of MPS-ODN with complementary RNA and their half-life evaluations against SVPDE287.
| ODN (5′ → 3′)a | T m with RNA (°C) | t 1/2 (s) |
|---|---|---|
| d(ACACCCAATT-CTGAAAATGG) | 51.2 | 44 |
| d(ACACCCAATT-CTGAAAATMPSGMPSG) | 47.8 | 210 |
| d(ACACCCAATT-CTGAAAAMPSTMPSGMPSG) | 48 | 264 |
| d(ACACCCAATT-CTGAAAMPSAMPSTMPSGMPSG) | 47.1 | 401 |
MPS refers to the methylphosphonothioate internucleoside linkage.