Half-life evaluations of PS-ODN against Bal31 endonuclease and antisense activity evaluation in cultured T24 cells80.
| ODN (5′ → 3′)a | t 1/2 (min) | AS activityb (%) |
|---|---|---|
| d(CCACACCGACGGCGCCC) | 5 | 0 |
| d(CPSCPSAPSCPSAPSCPSC-GACGPSGPSCPSGPSCPSCPSC) | 8 | 35 |
| d(CPSCPSAPSCPSAPSCPSCPS-GACPSGPSGPSCPSGPSCPSCPSC) | 50 | 78 |
| [d(CCACACC-GACGGCGCCC)]-all-PS | >50 | 82 |
a
PS refers to the phosphorothioate internucleoside linkage.
b
Percentage of inhibition of Ha-ras mRNA expression by activation of RNase-H within the cells by the ODN tested at 0.1 μM.