. 2020 Dec 8;2(1):94–150. doi: 10.1039/d0cb00136h

Thermal denaturation studies (T_m values) of different ODN with complementary RNA and their degradation against mouse liver homogenates¹³⁷.

ODN (5′ → 3′)^a	T _m with RNA (°C)	Mouse liver homogenates^b	RNase-H activity^c	IC₅₀ (nM)
d(G^LMeC^LATTGGTATT^LMeC^LA^L)	59.4	—^d	5.8	93
d(G^LMeC^LA_3′SPTTGGTATT^LMeC^LA^L)	60.0	68	17.3	8833
d(G^LMeC^LA_5′SPTTGGTATT^LMeC^LA^L)	58.5	56	9.9	410
d(G^LMeC^LAT_3′SPTGGTATT^LMeC^LA^L)	62.5	34	13.6	936
d(G^LMeC^LAT_5′SPTGGTATT^LMeC^LA^L)	57.4	56	11.4	340
d(G^LMeC^LATTGG_3′SPTATT^LMeC^LA^L)	62.5	1.2	10.0	1345
d(G^LMeC^LATTGG_5′SPTATT^LMeC^LA^L)	58.5	41	11.3	—^d
d(G^LMeC^LATTGGTA_3′SPTT^LMeC^LA^L)	61.9	1.5	9.8	1129
d(G^LMeC^LATTGGTA_5′SPTT^LMeC^LA^L)	59.0	31	56.9	—^d

3′SP and 5′SP refer to the 3′-SP and the 5′-SP linkages respectively. L refers to LNA residues.

% intact gapmer at the end of the experiment.

% full length target RNA after 48 h of incubation.

Gapmer ODN not tested.

Thermal denaturation studies (Tm values) of different ODN with complementary RNA and their degradation against mouse liver homogenates137.