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(A) Tissue disks incubating in an air-liquid interface. (B) Schematic representation of biopsy punch and resulting disk. (C-E) H&E staining of parallel tissue sections. (F) Fluorescence microscopy using Filaggrin, Cav-1, and DAPI to delineate the stratum corneum, granulosum, spinosum, and basale of the epidermis.
