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Phase contrast, fluorescence, and secondary ion images of an unwounded region of skin tissue. Negative ion images of cholesterol sulfate, adenine, α-Tocopherol, FA 24:0, 25:0, 26:0 are of the form [M-H]-. The ion at 168.0 m/z is used here to represent the Sphingomyelin headgroup. A comparison between fluorescence and SIMS overlay demonstrates the fidelity of endogenous lipids as biomarkers for epidermal layers.
