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. Author manuscript; available in PMC: 2021 Aug 5.
Published in final edited form as: Cell Rep. 2021 Jun 29;35(13):109298. doi: 10.1016/j.celrep.2021.109298

Figure 1. Hepatic vagotomy protects against diet-induced hyperinsulinemia.

Figure 1.

(A) Visual operative field for hepatic vagotomy surgeries. Arrow A indicates the hepatic branch of the vagus, which was severed to vagotomize mice. Arrow A also indicates the electrode placement to record firing activity of the hepatic vagal afferent nerve (Figure 2F). Arrow B indicates where the hepatic vagal nerve was cut after securing the electrode to eliminate vagal efferent activity (Figure 2F).

(B–E) Effects of hepatic vagotomy on high-fat-diet (HFD)-induced weight gain (B), serum insulin (C), glucose (D), and glucose/insulin ratio (E) at 0 and 9 weeks.

(C–E) Asterisks denote significance (*p < 0.05) between bars of the same color.

(F) Regression of body weight and serum insulin concentrations during HFD feeding in sham and vagotomized mice.

(G–L) Effect of hepatic vagotomy after 9 weeks of HFD feeding on serum glucagon (G), oral glucose tolerance (OGTT; H), OGTT area under the curve (AUC; I), oral glucose-stimulated serum insulin (J), insulin tolerance (ITT; K), and ITT AUC (L).

Number below bar denotes n per group. All data are presented as mean ± SEM. NS, non-significant.