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. 2021 Jul 22;12:709344. doi: 10.3389/fpls.2021.709344

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Copyright © 2021 Margolin, Allen, Verbeek, van Diepen, Ximba, Chapman, Meyers, Williamson, Crispin and Rybicki.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of MARV GPΔTM glycosylation when the protein was expressed in plants and mammalian cells. The data represent the percentage point change in glycosylation when the protein was expressed in N. benthamiana compared to HEK293, so a positive value represents a category of glycan that is enriched on material produced in N. benthamiana. Glycans are categorized according to the detected compositions by LC-MS. Compositions containing Hex(10-12) HexNAc(2) were categorized as M9Glc1-3 and Hex(9-5) HexNAc(2) as M9-M5. These are classified as oligomannose-type glycans and are colored green. Hybrid-type glycans contain compositions consisting of Hex(5)HexNAc(3)X or Hex(6)HexNAc(4)X. Remaining glycan compositions were assigned as complex-type. Any composition Hex(3) HexNAc(2) or smaller is classified as truncated. The proportion of potential N-linked glycans which are classified as unocupied are coloured grey.