Figure 2.

Effects of developmental alcohol exposure on viability and behavioral phenotypes in adult flies. (A) Boxplots of viability (n = 12 reps of 50 embryos per treatment), (B) Ethanol sensitivity (n = 43–49, 3–5 day old flies per sex per treatment), (C) Activity, (D) Proportion of daytime sleep, (E) Activity bouts during the day. (F) Proportion of night time sleep, (G) Activity bouts during the night. Day hours are from 7 a.m. to 7 p.m., lights on 7 h after hour zero. Gray boxes indicate flies reared on medium supplemented with 10% (v/v) ethanol and white boxes indicate control flies grown on regular medium. n = 57–64 flies per sex per treatment for all sleep and activity phenotypes. *p < 0.05, **p < 0.01, ***p < 0.001. Actograms are shown in Supplementary Figure 1.