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. 2021 May 21;2(4):1096–1114. doi: 10.1039/d1cb00022e

Summary of the detection sensitivities of various DNA modifications analyzed by chemical derivatization-mass spectrometry. LOD, limit of detection.

Derivatization reagents Modifications LOD (fmol) Ref.
2-Bromo-1-(4-dimethyl aminophenyl)-ethanone (BDAPE) 5mC 0.10 98
5hmC 0.06
5fC 0.11
5caC 0.23
2-Bromo-1-(4-diethylaminophenyl)-ethanone (BDEPE) 5mC 0.06 99
5hmC 0.07
5fC 0.10
5caC 0.08
2-Bromo-1-(4-1-pyrrolidinylphenyl)-ethanone (BPPE) 5mC 0.43
5hmC 0.69
5fC 0.79
5caC 1.21
3-Bromoacetonyltrimethylammonium bromide (BTA) 5mC 2.20
5hmC 4.72
5fC 6.22
5caC 0.53
ω-Bromoacetonylpyridinium bromide (BPB) 5mC 5.25
5hmC 2.72
5fC 16.59
5caC 4.41
4-(Dimethylamino) benzoic anhydride 5mC 2.24 100
5hmC 2.53
5fC 2.54
5caC 1.27
Uridine diphosphoglucose (UDP-glucose) 5hmC 6.7 102
5gmC 1.5
Girard's T reagent 5fU 3.3 103
5fC 0.09 104
5caC 0.77
5fC 0.08 105
5fU 0.14
Girard's D reagent 5fC 0.15 104
5caC 0.42
Girard's P reagent 5fC 0.03 104
5caC 0.75
5fC 0.03 105
5fU 0.05
4-(2-(Trimethylammonio)ethoxy)benzenaminium halide (4-APC) 5fC 0.05 105
5fU 0.09
Hydrazino-s-triazine based glycan labelling reagents i-Pr2N 5fC 0.01 106
5caC 0.025
Hydrazino-s-triazine based glycan labelling reagents Me2N 5fC 0.05
Hydrazino-s-triazine based glycan labelling reagents Et2N 5fC 0.0125
Dansylhydrazine (DNSH) 5hmC 0.04 107
2-Bromoacetophenone 5mC 22.7 131