Fig. 1. Schematic representation of a Click-based amplification procedure with streptavidin-N3. (A) The target of interest is labelled with the azide. (B) The first set of biotin tags are introduced via click reaction. (C) Non-amplified control: streptavidin-AF647 binds to the biotin on the target for imaging. (D) Amplifier binding step. Streptavidin-N3 binds to the biotin tags on the target and introduces multiple azide groups. (E) Click conversion step. A second set of biotin is introduced via click reaction. (F) Amplified signal: the additional biotin tags recruit more streptavidin-AF647 and result in enhanced fluorescence.
