Fig. 6. Click-based amplification on fixed intestinal sections of mice treated with afatinib-N3. (A) Scheme of P.O. treatment of mice with afatinib and afatinib-N3. (B) Structure of afatinib (control) and afatinib-N3. (C) Top: confocal imaging of fixed intestinal sections without and with Click-based amplification. The mice were orally administered with afatinib (10.0 mg kg−1) or afatinib-N3 (11.4 mg kg−1, equal mol), and dissection was carried out 24 h later. DAPI stained the nuclei of the intestinal cells. Scale bars, 200 μm. Bottom: Quantification of tissue fluorescence intensities. The whole specimen was circled and measured for the mean fluorescence intensity with ImageJ. Blue column, tissue fluorescence intensity without Click-based amplification. Red column, tissue fluorescence intensity with Click-based amplification. (D) Amplification ratios (Amp./No Amp.). (E) Signal-to-noise ratios (afatinib-N3/afatinib).
