Table 1. The 10 amino acid differences between prM proteins of African and Asian ZIKV isolates.

Amino acid coloring corresponds with that used in Fig 7. SM, suckling mouse; ?, Unknown; NGS, next-generation sequencing.

Genotype	Strain	Accession number	Source	Country	Year	Passage history	pr								M
							3	17	21	26	31	35	36	31	45	47
AFRICAN	MR766	LC002520	Monkey	Uganda	1947	$146\times \mathrm{SM}$, 1 × C6/36, 1 × VeroE6	I	S	$\mathrm{K}$	A	V	$\mathrm{H}$	V	$\mathrm{K}$	V	V
	MP1751	KY288905	Mosquito	Uganda	1962	$3\times \mathrm{SM}$,? × VeroE6	I	S	$\mathrm{K}$	A	V	$\mathrm{H}$	V	$\mathrm{K}$	V	V
	IbH_30656	HQ234500	Human	Nigeria	1968	$21\times \mathrm{SM}$, 1 × Vero	I	S	$\mathrm{K}$	V	V	$\mathrm{H}$	V	$\mathrm{K}$	V	V
	ArB 1362	KF383115	Mosquito	Central African Republic	1968	Not available	I	S	$\mathrm{K}$	A	V	$\mathrm{H}$	V	$\mathrm{K}$	A	V
	ArD7117	KF383116	Mosquito	Senegal	1968	Not available	I	S	$\mathrm{K}$	A	V	$\mathrm{H}$	V	$\mathrm{K}$	V	V
	ARB13565	KF268948	Mosquito	Central African Republic	1976	Not available	I	S	$\mathrm{K}$	A	V	$\mathrm{H}$	V	$\mathrm{K}$	A	V
	ARB7701	KF268950	Mosquito	Central African Republic	1976	Not available	I	S	$\mathrm{K}$	A	V	$\mathrm{H}$	V	$\mathrm{K}$	A	V
	ARB15076	KF268949	Mosquito	Central African Republic	1980	Not available	I	S	$\mathrm{K}$	A	V	$\mathrm{H}$	V	$\mathrm{K}$	A	V
	ArD_41519	HQ234501	Mosquito	Senegal	1984	1 × AP61, 2 × C6/36	I	S	$\mathrm{K}$	A	V	$\mathrm{H}$	V	$\mathrm{K}$	V	V
	ArD_41525	KU955591	Mosquito	Senegal	1984	1 × AP61, 1 × C6/36, 3 × VeoE6	I	S	$\mathrm{K}$	A	V	$\mathrm{H}$	V	$\mathrm{K}$	V	V
	ArD_41524	KX601166	Mosquito	Senegal	1984	1 × AP61, 1 × C6/36, 2 × VeoE6	I	S	$\mathrm{K}$	A	V	$\mathrm{H}$	V	$\mathrm{K}$	V	V
	ArD128000	KF383117	Mosquito	Senegal	1997	Not available	I	S	$\mathrm{K}$	A	V	$\mathrm{H}$	V	$\mathrm{K}$	A	V
	ArD157995	KF383118	Mosquito	Senegal	2001	Not available	I	S	$\mathrm{K}$	A	V	$\mathrm{H}$	V	M	V	V
ASIAN	PRVABC59	KU501215	Human	Puerto Rico	2015	No passage. NGS of patient sample	V	N	E	$\mathrm{P}$	$\mathrm{M}$	$\mathrm{Y}$	I	R	A	A
	P6-740	HQ234499	Mosquito	Malaysia	1966	$6\times \mathrm{SM}$, 1 × Vero, 1 × BHK, 1 × C6/36	V	S	E	$\mathrm{P}$	V	$\mathrm{Y}$	I	R	A	A
	Yap/Micronesia	EU545988	Human	Micronesia	2007	No passage. Sequence only	V	S	E	$\mathrm{P}$	$\mathrm{M}$	$\mathrm{Y}$	I	R	A	A
	FSS13025	JN860885	Human	Cambodia	2010	1 × Vero	V	S	E	$\mathrm{P}$	$\mathrm{M}$	$\mathrm{Y}$	I	R	A	A
	H/PF/2013	KJ776791	Human	French Polynesia	2013	3 × Vero	V	N	E	$\mathrm{P}$	$\mathrm{M}$	$\mathrm{Y}$	I	R	A	A
	BeH819966	KU365779	Human	Brazil	2015	? × C6/36	V	N	E	$\mathrm{P}$	$\mathrm{M}$	$\mathrm{Y}$	I	R	A	A
	FLR	KU820897	Human	Colombia	2015	1 × C6/36	V	N	E	$\mathrm{P}$	$\mathrm{M}$	$\mathrm{Y}$	I	R	A	A
	Brazil-ZKV2015	KU497555	Human	Brazil	2015	No passage. NGS of amniotic fluid.	V	N	E	$\mathrm{P}$	$\mathrm{M}$	$\mathrm{Y}$	I	R	A	A
	Natal RGN	KU527068	Human	Brazil	2015	Not available	V	N	E	$\mathrm{P}$	$\mathrm{M}$	$\mathrm{Y}$	I	R	A	A
	BeH818305	KU729218	Human	Brazil	2015	? × C6/36	V	N	E	$\mathrm{P}$	$\mathrm{M}$	$\mathrm{Y}$	I	R	A	A
	Rio-U1	KU926309	Human	Brazil	2016	1 × VeroE6	V	N	E	$\mathrm{P}$	$\mathrm{M}$	$\mathrm{Y}$	I	R	A	A
	Rio-S1	KU926310	Human	Brazil	2016	1 × VeroE6	V	N	E	$\mathrm{P}$	$\mathrm{M}$	$\mathrm{Y}$	I	R	A	A
	ZIKVNL00013	KU937936	Human	Suriname	2016	Vero	V	N	E	$\mathrm{P}$	$\mathrm{M}$	$\mathrm{Y}$	I	R	A	A
	ZKA-16-291	KX827309	Human	Singapore	2016	Not available	V	S	E	$\mathrm{P}$	$\mathrm{M}$	$\mathrm{Y}$	I	R	A	A
	ZIKV/Hu/NIID123/2016	LC219720	Human	Vietnam	2016	1 × C6/36, 4 × Vero	V	S	E	$\mathrm{P}$	$\mathrm{M}$	H	I	R	A	A