KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Human-p16INK4A (D3W8G) antibody | Cell Signaling Technology | Cat#92803; RRID:AB_2750891 |
| Mouse-p16INK4A (M-156) antibody | Santa Cruz Biotechnology | Cat# sc-1207; RRID:AB_632106 |
| Phospho-Histone H2A.X (Ser139) (20E3), antibody | Cell Signaling Technology | Cat#9718; RRID: AB_2118009 |
| IL-6 (D5W4V) antibody | Cell Signaling Technology | Cat# 12912; RRID:AB_2687897 |
| Anti-HMGB1 antibody | Abcam | Cat#ab18256; RRID:AB_444360 |
| Anti-RAGE antibody | Abcam | Cat#ab37647; RRID:AB_777613 |
| Monoclonal Anti-β-Actin–Peroxidase antibody | Sigma | Cat#A3854; RRID:AB_262011 |
| Phospho-p38 MAPK (Thr180/Tyr182) (28B10) antibody | Cell Signaling Technology | Cat#9216; RRID:AB_331296 |
| p38 MAPK Antibody | Cell Signaling Technology | Cat#9212; RRID:AB_330713 |
| Phospho-NF-κB p65 (Ser536) (93H1) antibody | Cell Signaling Technology | Cat#3033; RRID:AB_331284 |
| Anti-GAPDH antibody | Abcam | Cat#ab9485; RRID:AB_307275 |
| Anti-GFAP antibody | Abcam | Cat#ab4674; RRID:AB_304558 |
| TauO specific monoclonal antibody (TOMA2) | Montalbano et al., 2020 | N/A |
| T22 antibody | In-house; Nilson et al., 2017 | N/A |
| AT8, Phospho-Tau (Ser202, Thr205) Antibody | Thermo | Cat#MN1020; RRID:AB_223647 |
| Anti-NeuN Antibody, clone A60 antibody | Chemicon | Cat#MAB377; RRID:AB_2298772 |
| Recombinant Alexa fluor488 anti-NeuN antibody | Abcam | Cat#ab190195, RRID:AB_2716282 |
| Anti-Synapsin I antibody (ab8) | Abcam | Cat#ab8; RRID:AB_2200097 |
| Anti-PSD95 antibody | Abcam | Cat#ab18258; RRID:AB_444362 |
| Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 | Invitrogen | Cat#A-11011; RRID:AB_143157 |
| Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 | Invitrogen | Cat#A-11034; RRID:AB_2576217 |
| Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 633 | Invitrogen | Cat#A-21052; RRID:AB_2535719 |
| Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) | Abcam | Cat#ab150169; RRID:AB_2636803 |
| HRP-conjugated anti-rabbit IgG | GE Healthcare | NA934-1ML; RRID:AB_772206 |
| HRP-conjugated anti-mouse IgG | GE Healthcare | NA93-11ML; RRID:AB_772210 |
| Biological samples | ||
| Human frontal cortex brain tissues from NDC, AD, and FTD patients | The Institute for Brain Aging and Dementia at UC Irvine Puangmalai et al., 2020; Farmer et al., 2020; Montalbano et al., 2020; Nilson et al., 2017 | N/A |
| Escherichia coli BL21(DE3) competent cells | Agilent | Cat# 200131 |
| Chemicals, peptides, and recombinant proteins | ||
| Bovine serum albumin (BSA) | Sigma | Cat# A4161 |
| DMEM | GIBCO | Cat# 11960-044 |
| Triton X-100 | Sigma | Cat# T8787 |
| Protease inhibitor cocktail | Sigma | Cat#p8340-1ml |
| Ethyl pyruvate | Sigma | Cat#8066170100 |
| Glycyrrhizic acid or glycyrrhizin | Sigma | Cat#50531 |
| HBSS | GIBCO | Cat#14175-095 |
| Formaldehyde solution | Sigma | Cat#F8775-500ML |
| ProLong Gold Antifade reagent | Thermo | Cat#P36935 |
| Fetal Bovine Serum | GIBCO | Cat#1600-044 |
| Goat serum | Cell Signaling Technology | Cat#5425 |
| TauO | Ghag et al., 2018; Puangmalai et al., 2020 | N/A |
| Recombinant HMGB1 | Zandarashvili et al., 2013 | N/A |
| DNase I | Biolabs | Cat#M0303S |
| Antibiotic-Antimycotic (100X) | GIBCO | 15240096 |
| Poly-L-lysine solution | Sigma | Cat#P4832 |
| AF568 Protein Labeling kit | Invitrogen | Cat# A10238 |
| PBS | Corning | Cat#46-013-CM |
| RIPA buffer | Cell Signaling Technology | Cat#9806 |
| Thioflavine S | Sigma | Cat# T1892 |
| Critical commercial assays | ||
| BCA protein assay kit | Pierce | Ca#23225 |
| Cellular Senescence Assay kit | Sigma | Cat#KAA002 |
| Cell cycle analysis: FxCycle™PI/RNase staining | Invitrogen | Cat#F10797 |
| Mouse IL-6 ELISA | Thermo | Cat#88-7064; |
| Mouse TNF-α ELISA | Thermo | Cat#88-7324; |
| HMGB1 ELISA | Novus Biologicals | Cat#NBP2-62766 |
| Cation exchange chromatography column | Cytiva | Cat# 29018183 |
| Superdex 200 Increase 10/300 GL | Cytiva | Cat# 28990944 |
| WesternBright ECL HRP substrate | Advanstra | Cat#K-12045-D50 |
| TrueBlack Lipofuscin Autofluorescence Quencher | Biotium | Cat#23007 |
| Deposited data | ||
| Raw immunoblotting images | This paper | https://dx.doi.org/10.17632/765jbfjc5k.1 |
| Experimental models: Organisms/strains | ||
| Mouse: C57LB/6J | Jackson Laboratory | Stock#000664 |
| Mouse: hTau | Jackson Laboratory | Stock#004808 |
| Software and algorithms | ||
| ImageJ | NIH | https://imagej.nih.gov/ij/ |
| BZ-X Analyzer | Keyence | https://www.keyence.com |
| LSRII Fortessa Analyzer | BD Biosciences | N/A |
| ANY-maze Behavioral tracking software | ANY-maze | https://www.anymaze.co.uk/index.htm |
| GraphPad Prism 6 | Prism - GraphPad | https://www.graphpad.com |