Fig. 4. Accumulation of roxi-C4-Tz-NBD 9 in susceptible S. aureus (MSSA476) (A–C) and E. coli (BW25113) (D–F) cells as monitored by single-cell microfluidics over time. Fluorescence of individual cells is tracked in (A and D), with the average shown in bold with symbols. Times of snapshots (B, C and E, F) are indicated with dashed vertical lines. Brightfield (B and E) and the corresponding fluorescent (C and F) images are shown at early (2200 and 4000 s) and late (4400 and 12 000 s) timepoints for both species.
