Fig. 2. Step-wise modification of Magacizumab by PD 1 to form the antibody-fluorophore conjugate (AFC) 3. (a) Conditions for generation of Magacizumab-Alexa FluorTM 488 conjugate 3 (Maga-488 3); (b) SDS-PAGE analysis. M – marker, Lane 1 – reduced Magacizumab, Lane 2 – Rebridged Magacizumab 2a at 21 °C, Lane 3 – Rebridged Magacizumab 2b at 4 °C, Lane 4 – AFC 3a at 21 °C, Lane 5 – AFC 3b at 4 °C; (c) ELISA showing binding affinity of AFC 3a and Magacizumab to hLRG1; (d) serum stability analysis of AFC 3a by HPLC-SEC.
