Fig. 3. Generation of a stable B16F0 cell line expressing hLRG1. (a and b) qPCR was used to determine expression levels of mouse Lrg1 (a) and human Lrg1 (b) in transfected and non-transfected cells. Expression values were normalised to expression of the housekeeping gene mouse gapdh. Data are shown as mean ± SEM. Experiments were performed in triplicate (n = 3) and statistical analysis performed using Student's t-test (ns = not significant, *** = P ≤ 0.001). (c) western blot analysis of hLRG1 expression in B16F0 cells. Supernatants were probed using a rabbit anti-hLRG1 antibody.
