Fig. 4. In vivo survival assay using Drosophila melanogaster infection model. Seven-day-old female kenny^C02831 flies were subjected to sterile injury (A) or septic injury (B) with a thin tungsten needle previously dipped in sterile PBS alone or in an E. coli suspension diluted in PBS and kept at 29 °C. 1 and 24 hours after injury, 18.4 nL of peptides (1 mM) diluted in PBS or PBS alone were injected into the flies body cavity. Data represents means ± standard errors of 3 independent experiments. Individual experiments and their respective log-rank analysis are presented in SI.5 (ESI†). The survival rate at 144 hours (C) and the Lethal Time 50% (LT50) (D) represent the mean ± standard errors of the value determined for each of the 3 independent experiments and data were analyzed by the One-Way ANOVA test: *P < 0.05, **P < 0.01, ***P < 0.001. ns: not significant.