Peptides used in this study and their affinity to the ECSC and EC70S ribosome targets.
| Name | Sequencea | M w | Targets | K D (nM) ± sdb |
|---|---|---|---|---|
| P 7 | Ac-QXDLF-OH | 716.82 | EcSC | 194 ± 58 |
| Ec70S ribosome | ni | |||
| Py | VDKGSYLPRPTPPRPIYNRN-NH2 | 2339.70 | EcSC | ni |
| Ec70S ribosome | 15 ± 10 | |||
| Py-P 7 | VDKGSYLPRPTPPRPIYNRNGPRQXDLF-OH | 3307.82 | EcSC | 120 ± 20 |
| Ec70S ribosome | 37 ± 0.3 | |||
| Py-P 7Scr | VDKGSYLPRPTPPRPIYNRNGPRXFQLD-OH | 3307.82 | EcSC | ni |
| Ec70S ribosome | 76 ± 20 | |||
| FITC-Py | FITC-KVDKGSYLPRPTPPRPIYNRN-NH2 | 2826.17 | EcSC | ni |
| Ec70S ribosome | >104 | |||
| FITC-Py-P7 | FITC-KVDKGSYLPRPTPPRPIYNRNGPRQXDLF-OH | 3794.30 | EcSC | 300 ± 30 |
| Ec70S ribosome | >104 | |||
| FITC-Py-P7Scr | FITC-KVDKGSYLPRPTPPRPIYNRNGPRXFQLD-OH | 3794.30 | EcSC | ni |
| Ec70S ribosome | >104 | |||
| Onc112 | VDKPPYLPRPRPPRrIYNr-NH2 | 2389.85 | EcSC | ni |
| Ec70S ribosome | 84 ± 20 | |||
| Onc112-P 7 | VDKPPYLPRPRPPRrIYNrNGPRQXDLF-OH | 3472.08 | EcSC | 200 ± 0.1 |
| Ec70S ribosome | 70 ± 10 | |||
| Onc112-P 7Scr | VDKPPYLPRPRPPRrIYNrNGPRXFQLD-OH | 3472.08 | EcSC | ni |
| Ec70S ribosome | 100 ± 0.9 |
a
X = Cha: cyclohexylalanine. r: (d)-R amino acid. Scr: scramble. FITC: fluorescein isothiocyanate. Pyrrhocoricin (Py) was purchased from Novopro. For all peptides the purity, as determined by HPLC (λ = 214 nm), was ≥98%.
b
K D for each target were determined by ITC at 30 °C. Data are means ± sd of at least 2 independent experiments (see Table SI.4, ESI). ni: no specific interaction (see SI.2–4, ESI for full thermodynamic data and analysis).