Fig. 4. Representative workflow established by Going et al.115 As quantitative proteomics is critical for discovering and validated biomolecules of interest during periods of disease and treatment, this workflow represents an example of how treatment strategies may be controlled and systematically evaluated. While SILAC methods would be useful in situations where cell growth is monitored, isotopic labeling methods may be considered inherently lower throughput due to the increases in spectral complexity they may provide. Reprinted with permission.
