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. 2021 Jul 26;10:e67390. doi: 10.7554/eLife.67390

Table 3. Yeast strains.

Strain name Relevant genotype Source
SWY355 Mat a; tor1-1, fpr1::loxP-LEU2-loxP, RPL13A-2xFKBP12::loxP-TRP1-loxP Haruki et al., 2008
SWY536 Mat a; tor1-1 fpr1::loxP-LEU2-loxP RPL13A-2xFKBP12::loxP, Ame1-FRB::KanMX Hornung et al., 2014
PSY1.1 Mat a; tor1-1 fpr1::loxP-LEU2-loxP RPL13A-2xFKBP12::loxP, Mtw1-FRB::KanMX Killinger et al., 2020
MLY3 Mat a; tor1-1 fpr1::loxP-LEU2-loxP RPL13A-2xFKBP12::loxP, Ame1-FRB::KanMX, Ame1-WT-6xFlag::URA3 This study
MLY5 Mat a; tor1-1 fpr1::loxP-LEU2-loxP RPL13A-2xFKBP12::loxP, Ame1-FRB::KanMX, Ame1-7A-6xFlag::URA3 This study
MLY15 Mat a, ade2-1, leu2-3,112, his3Δ200, ame1Δ::HIS3, ura3-52, Ame1-WT-6xFlag::URA3 This study
MLY31 Mat a; tor1-1 fpr1::loxP-LEU2-loxP RPL13A-2xFKBP12::loxP, Ame1-FRB::KanMX, Ame1-7E-6xFlag::URA3 This study
MBY79 Mat a, ade2-1, his3Δ200, ame1Δ::HIS3, leu2-3,112, ura3-52, Ame1-WT-6xFlag::URA3, Cse4-13xMyc::KanMX This study
MBY81 Mat a, ade2-1, his3Δ200, ame1Δ::HIS3, leu2-3,112, ura3-52, Ame1-7E-6xFlag::URA3, Cse4-13xMyc::KanMX This study
MBY83 Mat a, ade2-1, his3Δ200, ame1Δ::HIS3, leu2-3,112, ura3-52, Ame1-7A-6xFlag::URA3, Cse4-13xMyc::KanMX This study
MBY153 Mat α, lys2-801am, leu2-3,112, his3Δ200, ura3-52 (two-micron-pESC-HIS) (pGAL-empty) This study
MBY155 Mat α, lys2-801am, leu2-3,112, his3Δ200, ura3-52 (two-micron pESC-HIS-Ame1-WT-1xFlag + Okp1-WT-1xMyc) This study
MBY156 Mat α, lys2-801am, leu2-3,112, his3Δ200, ura3-52 (two-micron pESC-HIS-Ame1-7A-1xFlag + Okp1-WT-1xMyc) This study
MBY157 Mat α, lys2-801am, leu2-3,112, his3Δ200, ura3-52 (two-micron pESC-HIS-Ame1-7E-1xFlag + Okp1-WT-1xMyc) This study
MBY158 Mat α, lys2-801am, leu2-3,112, his3Δ200, ura3-52, psh1Δ::NatNT2 (two-micron pESC-HIS) This study
MBY160 Mat α, lys2-801am, leu2-3,112, his3Δ200, ura3-52, psh1Δ::NatNT2 (two-micron pESC-HIS-Ame1-WT-1xFlag + Okp1-WT-1xMyc) This study
MBY161 Mat α, lys2-801am, leu2-3,112, his3Δ200, ura3-52, psh1Δ::NatNT2 (two-micron pESC-HIS-Ame1-7A-1xFlag + Okp1-WT-1xMyc) This study
MBY162 Mat α, lys2-801am, leu2-3,112, his3Δ200, ura3-52, psh1Δ::NatNT2 (two-micron pESC-HIS-Ame1-7E-1xFlag + Okp1-WT-1xMyc) This study
MBY163 Mat α, lys2-801am, leu2-3,112, his3Δ200, ura3-52, mad1Δ::KanMX (two-micron pESC-HIS) This study
MBY165 Mat α, lys2-801am, leu2-3,112, his3Δ200, ura3-52, mad1Δ::KanMX (two-micron pESC-HIS-Ame1-WT-1xFlag + Okp1-WT-1xMyc) This study
MBY166 Mat α, lys2-801am, leu2-3,112, his3Δ200, ura3-52, mad1Δ::KanMX (two-micron pESC-HIS-Ame1-7A-1xFlag + Okp1-WT-1xMyc) This study
MBY167 Mat α, lys2-801am, leu2-3,112, his3Δ200, ura3-52, mad1Δ::KanMX (two-micron pESC-HIS-Ame1-7E-1xFlag + Okp1-WT-1xMyc) This study
MBY225 Mat α, his3Δ200, ura3-52, Ame1-TM2-6xFlag::URA3, lys2-801am, tor1-1 fpr1::loxP-LEU2-loxP RPL13A-2xFKBP12::loxP-TRP1-loxP, Ame1-FRB::KanMX This study
MBY226 Mat α, his3Δ200, ura3-52, Ame1-TM3-6xFlag::URA3, lys2-801am, tor1-1 fpr1::loxP-LEU2-loxP RPL13A-2xFKBP12::loxP-TRP1-loxP, Ame1-FRB::KanMX This study
MBY227 Mat α, his3Δ200, ura3-52, Ame1-TM4-6xFlag::URA3, lys2-801am, tor1-1 fpr1::loxP-LEU2-loxP RPL13A-2xFKBP12::loxP-TRP1-loxP, Ame1-FRB::KanMX This study
MBY228 Mat α, his3Δ200, ura3-52, Ame1-TM5-6xFlag::URA3, lys2-801am, tor1-1 fpr1::loxP-LEU2-loxP RPL13A-2xFKBP12::loxP-TRP1-loxP, Ame1-FRB::KanMX This study
MBY241 Mat α, lys2-801am, leu2-3,112, his3Δ200, ura3-52 (two-micron pESC-HIS-Ame1-S52A+S53A-1xFlag + Okp1-WT-1xMyc) This study
MBY255 Mat α, lys2-801am, leu2-3,112, his3Δ200, ura3-52 (two-micron pESC-HIS-Ame1-ILTPP-1xFlag + Okp1-WT-1xMyc) This study
MBY256 Mat α, lys2-801am, leu2-3,112, his3Δ200, ura3-52 (two-micron pESC-HIS-Ame1-5A+ILTPP-1xFlag + Okp1-WT-1xMyc) This study
MBY273 Mat a, ade2-1, leu2-3,112, his3Δ200, ame1Δ::HIS3, ura3-52, Ame1-WT-CPDILTPP-6xFlag::URA3 This study
MBY275 Mat α, lys2-801am, leu2-3,112, his3Δ200, ura3-52 (two-micron pESC-HIS-Ame1-T31A-1xFlag + Okp1-WT-1xMyc) This study
MBY276 Mat α, lys2-801am, leu2-3,112, his3Δ200, ura3-52 (two-micron pESC-HIS-Ame1-S45A-1xFlag + Okp1-WT-1xMyc) This study
MBY278 Mat α, lys2-801am, leu2-3,112, his3Δ200, ura3-52 (two-micron pESC-HIS-Ame1-S41A+S45A-1xFlag + Okp1-WT-1xMyc) This study
MBY279 Mat α, lys2-801am, leu2-3,112, his3Δ200, ura3-52 (two-micron pESC-HIS-Ame1-S41A-1xFlag + Okp1-WT-1xMyc) This study
MBY292 Mat a, ura3-52, lys2-801, ade2-101, his3Δ200, trp1Δ63, leu2Δ1, skp1-3::LEU2 (two-micron pESC-HIS-Ame1-WT-1xFlag-Okp1-WT-1xMyc) This study
MBY293 Mat a, ura3-52, lys2-801, ade2-101, his3Δ200, trp1Δ63, leu2Δ1, skp1-3::LEU2 (two-micron pESC-HIS-Ame1-7A-1xFlag-Okp1-WT-1xMyc) This study
MBY310 Mat a, cdc53-1 (ts), ade2-1, trp1-1, can1-100, leu2-3,112, his3-11,15, ura3, psi+, ssd1-d2 (two-micron pESC-HIS-Ame1-WT-1xFlag-Okp1-WT-1xMyc) This study
MBY311 Mat a, cdc53-1 (ts), ade2-1, trp1-1, can1-100, leu2-3,112, his3-11,15, ura3, psi+, ssd1-d2 (two-micron pESC-HIS-Ame1-7A-1xFlag-Okp1-WT-1xMyc) This study
MBY312 Mat a, cdc34-2 (ts), ade2-1, trp1-1, can1-100, leu2-3,112, his3-11,15, ura3, psi+, ssd1-d2 (two-micron pESC-HIS-Ame1-WT-1xFlag-Okp1-WT-1xMyc) This study
MBY313 Mat a, cdc34-2 (ts), ade2-1, trp1-1, can1-100, leu2-3,112, his3-11,15, ura3, psi+, ssd1-d2 (two-micron pESC-HIS-Ame1-7A-1xFlag-Okp1-WT-1xMyc) This study
MBY314 Mat α, cdc4-1 (ts), ade2-1, trp1-1, can1-100, leu2-3,112, his3-11,15, ura3, psi+, ssd1-d2 (two-micron pESC-HIS-Ame1-WT-1xFlag-Okp1-WT-1xMyc) This study
MBY315 Mat alpha, cdc4-1 (ts), ade2-1, trp1-1, can1-100, leu2-3,112, his3-11,15, ura3, psi+, ssd1-d2 (two-micron pESC-HIS-Ame1-7A-1xFlag-Okp1-WT-1xMyc) This study
MBY316 Mata, ade2-1, his3Δ200, trp1-1, ura3-52, grr1Δ::LEU2 (ts), lys2-801 (two-micron pESC-HIS-Ame1-WT-1xFlag-Okp1-WT-1xMyc) This study
MBY317 Mata, ade2-1, his3Δ200, trp1-1, ura3-52, grr1Δ::LEU2 (ts), lys2-801 (two-micron pESC-HIS-Ame1-7A-1xFlag-Okp1-WT-1xMyc) This study
MBY322 Mat a, ade2-1, leu2-3,112, his3Δ200, ura3-52, mub1Δ::natNT2 (two-micron pESC-HIS-Ame1-WT-1xFlag-Okp1-WT-1xMyc) This study
MBY323 Mat a, ade2-1, leu2-3,112, his3Δ200, ura3-52, mub1Δ::natNT2 (two-micron pESC-HIS-Ame1-7A-1xFlag-Okp1-WT-1xMyc) This study
MBY324 Mat a, his3Δ200, ame1Δ::HIS3, ura3-52, Ame1-3A-6xFlag::URA3, leu2-3-112 This study
MBY327 Mat a, his3Δ200, ame1Δ::HIS3, ura3-52, Ame1-4A-6xFlag::URA3, leu2-3-112 This study
MBY331 Mat α, lys2-801am, leu2-3,112, his3Δ200, ura3-52 (two-micron pESC-HIS-Ame1-7A-1xFlag+Okp1-1A-1xMyc) This study
MBY333 Mat a, ura3-52, lys2-801, ade2-101, his3Δ200, trp1Δ63, leu2Δ1, skp1-3::LEU2 (two-micron pESC-HIS-Ame1-7A-1xFlag+Okp1-1A-1xMyc) This study
MBY345 Mat a, ame1Δ::HIS3, ura3-52, Ame1-5A1::URA3, ade2-3, leu2-3,112 This study
MBY347 Mat a, ame1Δ::HIS3, ura3-52, Ame1-5A2::URA3, ade2-3, leu2-3,112 This study
MBY360 Mat α, cdc4-1, ade2-1, trp1-1, can1-100, leu2-3,112, his3-11,15, ura3, psi+, ssd1-d2 (two-micron pESC-HIS-Ame1-CPDILTPP-1xFlag+Okp1-WT-1xMyc) This study
MBY361 Mat α, cdc4-1, ade2-1, trp1-1, can1-100, leu2-3,112, his3-11,15, ura3, psi+, ssd1-d2 (two-micron pESC-HIS-Ame1-5A-CPDILTPP-1xFlag+Okp1-WT-1xMyc) This study
MBY371 Mat a, his3Δ200, ame1Δ::HIS3, ura3-52, Ame1-3A-6xFlag::URA3, leu2-3,112, Pds1-13xMyc::LEU2 This study
MBY372 Mat α, his3Δ200, ame1Δ::HIS3, ura3-52, Ame1-4A-6xFlag::URA3, leu2-3,112, Pds1-13xMyc::LEU2 This study
MBY373 Mat a, his3Δ200, ame1Δ::HIS3, ura3-52, Ame1-5A1::URA3, ade2-3, leu2-3,112, Pds1-13xMyc::LEU2 This study
MBY374 Mat a, his3Δ200, ame1Δ::HIS3, ura3-52, Ame1-5A2::URA3, ade2-3, leu2-3,112, Pds1-13xMyc::LEU2 This study
MBY380 Mat a, his3Δ200, ame1Δ::HIS3, ura3-52, Ame1-3A-6xFlag::URA3, lys2-801, trp1-1, leu2-3,112, skp1-3::LEU2 This study
MBY412 Mat α, cdc34-2, ade2-1, trp1-1, can1-100, leu2-3,112, his3-11,15, ura3, psi+, ssd1-d2 (two-micron pESC-HIS-Ame1-7A-1xFlag+Okp1-1A-1xMyc) This study
MBY434 Mat a, ade2-1, leu2-3,112, his3-11-15, ame1Δ::HIS3, ura3-52, Ame1-WT-CPDILTPP-6xFlag::URA3, psi1x, ssd1-d2, cdc4-1 This study
MBY456 Mat α, lys2-801am, leu2-3,112, his3Δ200, ura3-52 (two-micron pESC-HIS, two-micron pESC-URA3) This study
MBY457 Mat α, lys2-801am, leu2-3,112, his3Δ200, ura3-52 (two-micron pESC-HIS, two-micron pGAL-URA-Mcm21-WT-3xHA+Ctf19-WT-1xMyc) This study
MBY462 Mat α, lys2-801am, leu2-3,112, his3Δ200, ura3-52 (two-micron pESC-HIS-Ame1-WT-1xFlag+Okp1-WT-1xMyc, two-micron pESC-URA) This study
MBY463 Mat α, lys2-801am, leu2-3,112, his3Δ200, ura3-52 (two-micron pESC-HIS-Ame1-WT-1xFlag+Okp1-WT-1xMyc, two-micron pESC-URA-Mcm21-WT-3xHA+Ctf19-WT-1xMyc) This study
MBY466 Mat α, lys2-801am, leu2-3,112, his3Δ200, ura3-52 (two-micron pESC-HIS-Ame1-7A-1xFlag+Okp1-1A-1xMyc, two-micron pESC-URA) This study
MBY485 Mat α, his3Δ200, ura3-52, Ame1-3A-6xFlag::URA3, lys2-801am, tor1-1 fpr1::loxP-LEU2-loxP RPL13A-2xFKBP12::loxP-TRP1-loxP, Mtw1-FRB::KanMX This study
MBY486 Mat α, his3Δ200, ura3-52, Ame1-4A-6xFlag::URA3, lys2-801am, tor1-1 fpr1::loxP-LEU2-loxP RPL13A-2xFKBP12::loxP-TRP1-loxP, Mtw1-FRB::KanMX This study
MBY489 Mat a, ade2-1, leu2-3,112, his3Δ200, ura3-52, Ame1-3A-ΔN-6xFlag::URA3 This study
MBY490 Mat a, ade2-1, leu2-3,112, his3Δ200, ura3-52, Ame1-4A-ΔN-6xFlag::URA3 This study
MBY493 Mat a, ade2-101, his3Δ200, trp1Δ63, lys2-801am, leu2Δ1, skp1-3::LEU2, ura3-52, Ame1-3A-ΔN-6xFlag::URA3 This study
MBY494 Mat a, ade2-101, his3Δ200, trp1Δ63, lys2-801am, leu2Δ1, skp1-3::LEU2, ura3-52, Ame1-4A-ΔN-6xFlag::URA3 This study
MBY505.1 Mat a, his3Δ200, ame1Δ::HIS3, ura3-52, Ame1-WT-6xFlag::URA3, ade2-1, lys2-801am, leu2-3,112, ctf19Δ::natNT2 This study
MBY505.2 Mat a, his3Δ200, ame1Δ::HIS3, ura3-52, Ame1-WT-6xFlag::URA3, ade2-1, leu2-3,112, ctf19Δ::natNT2 This study
MBY509.2 Mat a, his3Δ200, ame1Δ::HIS3, ura3-52, Ame1-CPDILTPP-6xFlag::URA3, leu2-3,112, ctf19Δ::natNT2 This study
MBY510.1 Mat α, his3Δ200, ame1Δ::HIS3, ura3-52, Ame1-CPDILTPP-6xFlag::URA3, ade2-1, lys2-801am, leu2-3,112, ctf19Δ::natNT2 This study