Table 2. Number of SGFP2-CTNNB1 molecules in the confocal volume and corresponding calculated concentration of SGFP2-CTNNB1 molecules with the fast or slow diffusion coefficient obtained from FCS measurements in n=3 independent experiments.

The concentration is calculated from the number of molecules and the calibrated confocal volume (see Materials and methods).

			Fast SGFP2-CTNNB1				Slow SGFP2-CTNNB1
			Number of molecules		Concentration (nM)		Number of molecules		Concentration (nM)
Compartment	Treatment	n	Median	95% CI	Median	95% CI	Median	95% CI	Median	95% CI
Cytoplasm	BSA	21	51	40–63	91	66–139	29	20–37	57	38–76
	WNT3A	21	60	47–80	145	76–168	35	30–41	68	57–76
Nucleus	BSA	21	48	41–66	87	78–119	13	2–22	22	4–40
	WNT3A	18	96	81–101	170	147–214	47	37–49	86	64–104