Table 3. Brightness of SGFP2-compared relative to EGFP-monomer and -dimer controls in n=2 independent experiments. N is the number of analyzed cells.
p-Values were calculated using PlotsOfDifferences that uses a randomization test (Goedhart, 2019).Note that only the EGFP-dimer is significantly different to the EGFP-monomer control, while SGFP2-CTNNB1 is not.
| Fluorophore | Compartment | Treatment | N | Median | 95 CI median | p-value to matched control (EGFP monomer in the nucleus or cytoplasm) |
|---|---|---|---|---|---|---|
| EGFP-monomer | Cytoplasm | NA | 15 | 1 | 0.79–1.34 | 1.000 |
| EGFP-dimer | Cytoplasm | NA | 14 | 1.4 | 1.29–1.60 | 0.011* |
| SGFP2-CTNNB1 | Cytoplasm | BSA | 69 | 0.92 | 0.83–1.00 | 0.738 |
| SGFP2-CTNNB1 | Cytoplasm | 100 ng/ml WNT3A | 46 | 1.01 | 0.93–1.11 | 0.919 |
| EGFP-monomer | Nucleus | NA | 15 | 1 | 0.91–1.07 | 1.000 |
| EGFP-dimer | Nucleus | NA | 14 | 1.62 | 1.44–1.69 | <0.001* |
| SGFP2-CTNNB1 | Nucleus | BSA | 69 | 0.87 | 0.78–0.96 | 0.192 |
| SGFP2-CTNNB1 | Nucleus | 100 ng/ml WNT3A | 46 | 1.05 | 0.95–1.15 | 0.578 |