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. 2021 Jul 22;10:e68955. doi: 10.7554/eLife.68955

Figure 3. Fluorescence resonance energy transfer (FRET) is stronger within subunits than between subunits.

(A) Example cells transfected with the indicated constructs showing intensity (left) and lifetime (right) images. (B) Time-correlated single photon counting (TCSPC) histograms from the plasma membrane of cells in A and additional control. Solid line is a single exponential fit to the data. (C) Cartoon (upper) and lifetimes (lower) from the indicated constructs for measuring intra-subunit FRET. (D) Cartoon (upper) and lifetimes (lower) for measuring inter-subunit FRET. Symbols denote single cells (N = 35–76 cells) and error bars are SEM.

Figure 3.

Figure 3—figure supplement 1. Fluorescence resonance energy transfer (FRET) due to membrane ‘crowding’.

Figure 3—figure supplement 1.

Cartoon (upper) and lifetimes (lower) from the indicated constructs for measuring FRET between cASIC dimer and membrane tethered YFP (Lck-YFP) over varying cDNA ratios. Symbols denote single cells (N = 13–74 cells per construct) and error bars are SEM.