Fig. 2. Pressure dependent smFRET-histogram (A and C) and population distribution (B and D) of the folded (red) and partially folded (blue) conformation of the i-motif (∼50 pM) in 20 mM Tris HCl, pH 7.5, at 25 °C. The samples for C and D contained additional 100 μM α-Syn oligomers.