Fig. 1. Confocal fluorescence scanning images arising from the incubation of unloaded Ni²⁺NTA resin or survivin-loaded CONA resin (Scheme 1) with 200 pmol (200 μL of 1 μM stock) of His₆-survivin with extracts BRA 177 and X0192. (a) Brightfield (BF) and fluorescence (FL) images are shown for experiments using Ni²⁺NTA resin without survivin. (b) BF and FL images are shown for experiments with CONA resin. Two extract dilutions 1000× (high dilution) and 200× (low dilution) were compared to show fluorescence correlates with concentration. Fluorescence (FL) in both panels was collected by excitation at 561 nm and collecting emission at 585 nm. Fluorescence was not observed in control experiments using a non IAP protein, NusB/E (see Fig. S2, ESI†).