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. 2018 Nov 5;8(21):e3072. doi: 10.21769/BioProtoc.3072

Figure 2. Data analysis.

Figure 2.

A. Relative Myc mRNA abundance (2(-CT)) for each time point for 2 replicates in control and SRSF3 depleted iPS cell samples. B. One phase decay analysis in GraphPad Prism. C. The dissociation curve with a single peak indicating the amplification of a single PCR product. D. Graph representing Myc mRNA decay in control and SRSF3-knockout (KO) cells after Actinomycin D treatment demonstrating similar half-lives for Myc mRNA in control and SRSF3-KO cells.