Reagent	For 1 ml final volume (store at -20 °C)	Final Concentration
Formamide	0.1 ml	10%
Competitor (10 mg/ml E. coli tRNA	0.1 ml	1 mg/ml
20% Dextran Sulfate	0.5 ml	10%
20 mg/ml BSA	10 µl	0.2 mg/ml
20x SSC	0.1 ml	2x
VRC 200 mM	10 µl	2 mM
Nuclease-free water	0.18 ml
Total Volume	1 ml