Figure 3. Sample ¹H NMR spectra of WTA and LTA.

A. WTA was liberated from peptidoglycan of wild-type S. aureus by NaOH hydrolysis causing loss of D-ala modifications. This WTA spectrum was obtained using the ZG pulse sequence without water suppression. B. LTA purified from wild-type S. aureus has very low levels of GlcNAc glycosylation but is highly D-alanylated. This LTA spectrum was obtained using the ZGPR pulse sequence with water suppression. These NMR spectra are adapted from published figures in Kho and Meredith (2018). Rbo = Ribitol; Gro = Glycerol.