Figure 1. Differentiation of hiPSCs and hESCs into MSCs by one-step derivation in feeder-free system.

A. Schematic diagram of efficient differentiating hiPSCs and hESCs into MSCs by MSC low glucose medium treatment and serial passages with trypsinization in feeder-free culture system. B. Representative morphology of cells at key phages during MSC derivation from hiPSCs and hESCs at Days -2, 0, 7, 14 and 21, as well as P2 of mature hiPSC-MSCs and hESC-MSCs. P2, Passage 2. Scale bars: 100 μm.