Table 1. Recommended volume of related reagents for different culture vessels.

Culture vessels	Approx. surface area (cm2)	Diluted Vitronectin XF	DPBS w/o Ca2+ and Mg2+	ReLeSR	TeSR-E8 medium
6-well	10 cm2/well	1 ml	2 ml	1 ml	2 ml
12-well	4 cm2/well	0.4 ml	1 ml	0.4 ml	1 ml
24-well	2 cm2/well	0.2 ml	0.5 ml	0.2 ml	0.5 ml
35-mm dish	10 cm2/dish	1 ml	2 ml	1 ml	2 ml
60-mm dish	20 cm2/dish	2 ml	4 ml	2 ml	4 ml
100-mm dish	60 cm2/dish	6 ml	12 ml	6 ml	12 ml
T25 flask	25 cm2/flask	3 ml	5 ml	3 ml	5 ml
T75 flask	75 cm2/flask	9 ml	15 ml	9 ml	15 ml