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. 2018 Dec 5;8(23):e3109. doi: 10.21769/BioProtoc.3109

Figure 2. Representation of how the plating may look when comparing wild-type cells to mutants.

Figure 2.

In Row 1, the wild-type and lacA strains are plated at a 10-2 dilution. The comK and ecsB strains are plated with undiluted culture. All plates in Row 1 contain the selectable antibiotic relevant to the marker present on the donor DNA. Row 2 is plated on LB plates with 10-6 dilutions. Each column is a different genotype. Column 1 is wild type, column 2 is comK (a gene necessary for transformation), column 3 is lacA (a gene not related to natural transformation), and column 4 is ecsB (putatively involved with natural transformation). No colonies formed on the undiluted comK selective plate, a few colonies are visible on the ecsB selective plate, and colony formation was at the level of wild type for lacA. CFU can also can be compared to wild type at this step.