8% Separation gel (ml)	Stacking gel (ml)
Acrylamide1	5.35	1
1.875 M Tris pH 8.8	4	0
0.6 M Tris pH 6.8	0	1.2
MilliQ water	10.4	3.7
10% SDS	0.2	0.06
20% APS	0.1	0.03
TEMED	0.02	0.012
1 30% Acrylamide with 0.8% Bisacrylamide (37.5:1)