Figure 5. Surgical set-up.

A. Anesthetized rat positioned on the mouth bar; the incision is made in whichever direction suits the handedness of the surgeon. B. Mouth bar positioned in retort stand and with Perspex rat support and bubble wrap to illustrate position of the rat. C. Surgical drapes positioned over the retort stand to create a continuous sterile surface between the linen napkin and the surgical drape positioned over the rat. The window in the drape allows access to the right eye and top of the skull. Sterile cotton wool and gauze on the left.